Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source "Turkish DeLight"
Künye
Atalay, N., Akcan, E. K., Gül, M., Ayan, E., Destan, E., KUZUCU, F. B. E., ... & Yildirim, G. (2023). Cryogenic X-ray crystallographic studies of biomacromolecules at Turkish Light Source? Turkish DeLight?. Turkish Journal of Biology, 47(1), 1-13.Özet
Abstract: X-ray crystallography is a robust and powerful structural biology technique that provides high-resolution atomic structures
of biomacromolecules. Scientists use this technique to unravel mechanistic and structural details of biological macromolecules (e.g.,
proteins, nucleic acids, protein complexes, protein-nucleic acid complexes, or large biological compartments). Since its inception,
single-crystal cryocrystallography has never been performed in Türkiye due to the lack of a single-crystal X-ray diffractometer. The
X-ray diffraction facility recently established at the University of Health Sciences, İstanbul, Türkiye will enable Turkish and international
researchers to easily perform high-resolution structural analysis of biomacromolecules from single crystals. Here, we describe the
technical and practical outlook of a state-of-the-art home-source X-ray, using lysozyme as a model protein. The methods and practice
described in this article can be applied to any bio